01Target & sequence review
Define the organism or sequence to detect, review specificity requirements and select an appropriate molecular platform.
Radetec / Contract R&D / Melbourne
PCR, qPCR & LAMP Assay Development Australia
Develop PCR, qPCR and LAMP assays for pathogen detection, with sample preparation and the intended instrument considered from the start.
Development scope
From the first question
to the next milestone.
02Primer & reaction optimisation
Evaluate candidate primers and reaction conditions using suitable reference materials and controls.
03Sample preparation
Assess extraction or lysis methods, matrix inhibition and compatibility with the amplification chemistry.
04Workflow & verification
Evaluate the complete workflow, including control strategy, readout and analytical performance against project criteria.
Technical perspective
What matters in development
Molecular assay development starts with a clearly defined detection target and a sample-to-result workflow. Primer design alone is not enough: lysis, inhibition, controls and readout must be tested with the intended sample material and instrument.
Common project questions
How do you choose between PCR and LAMP?
We compare the intended instrument, sample preparation, turnaround target and specificity requirements before selecting the platform for evaluation.
Can sample preparation be included?
Yes. We can assess lysis or extraction approaches, matrix inhibition and their compatibility with the amplification chemistry.
Let's find your starting point
Have a project in mind?
Share your assay requirements or formulation goals and current stage. We'll discuss how to structure the next step.
Start a conversation