01Target & format review
Review the analyte, sample matrix, available reagents and measurement range to choose an appropriate ELISA format.
Radetec / Contract R&D / Melbourne
Custom ELISA Development Services Australia
Develop an enzyme-linked immunosorbent assay around your biomarker, sample matrix and required detection range.
Development scope
From the first question
to the next milestone.
02Reagent evaluation
Evaluate recognition reagents, standards and detection chemistry to establish a suitable starting assay.
03Assay optimisation
Refine coating, blocking, incubation, washing and detection conditions. Assess sample dilution and matrix effects.
04Analytical evaluation
Evaluate detection range, precision and specificity against agreed criteria, with recovery and dilution studies where appropriate.
Technical perspective
What matters in development
A useful ELISA needs more than a measurable signal. We consider antibody pairing, calibrators, sample dilution, matrix interference and the intended concentration range while developing the assay protocol.
Common project questions
Can you work with antibodies we already have?
Yes. We can evaluate available antibodies and standards as a starting point, then identify gaps that affect feasibility or performance.
Can the assay be developed for our sample matrix?
Sample matrix and dilution are part of the development brief. Recovery, background and matrix effects can be evaluated against agreed criteria.
Let's find your starting point
Have a project in mind?
Share your assay requirements or formulation goals and current stage. We'll discuss how to structure the next step.
Start a conversation