01Review the evidence
Assess existing protocols, performance data, reagents and the intended use to identify likely sources of variability.
Radetec / Contract R&D / Melbourne
Diagnostic Assay Optimisation & Troubleshooting
A focused investigation into the factors limiting sensitivity, specificity or consistency, followed by practical, evidence-led improvements.
Development scope
From the first question
to the next milestone.
02Design focused experiments
Prioritise a small set of variables and measurable acceptance criteria before starting bench work.
03Optimise the assay system
Study reagent loading, buffers, blocking, reaction conditions or sample handling as appropriate to the platform.
04Confirm the improvement
Compare the revised workflow with the baseline and document remaining limitations and next steps.
Technical perspective
What matters in development
When an assay is partly working, the fastest useful step is often a focused comparison against the current protocol. We review existing data, identify the variables most likely to limit performance and agree how improvements will be measured.
Common project questions
Do we need to start development again?
Usually we begin with your current reagents, protocol and data. The scope can focus on the specific bottleneck instead of repeating the whole development process.
What do you measure during optimisation?
Measures depend on the assay and project, but may include detection range, background, recovery, precision, matrix effects and run-to-run variation.
Let's find your starting point
Have a project in mind?
Share your assay requirements or formulation goals and current stage. We'll discuss how to structure the next step.
Start a conversation